A new ratiometric fluorescent probe for sensing HOC1 based on TBET in real time

作者：Song, Guang-Jie; Ma, Han-Lin; Luo, Jing; Cao, Xiao-Qun^*; Zhao, Bao-Xiang^*

来源：Dyes and Pigments, 2018, 148: 206-211.

DOI：10.1016/j.dyepig.2017.09.022

• Ratiometric fluorescent probe
• Hypochlorous acid
• TBET
• Cell imaging
• Real-time monitor

A new ratiometric fluorescent probe (RPM) was developed based on through-bond energy transfer (TBET) mechanism for detecting hypochlorous acid. The probe was constructed from imidazo[1,5-a] pyridine as the energy donor and the rhodamine as the energy acceptor. The probe could be used to determine the concentration of HOCI with high selectivity and sensitivity (the detection limit = 2.08 x 10(-6) M). In addition, RPM had a good time resolution for measuring HOCI with the response time of less than 30 s. Upon addition of HOCI, the fluorescence colors of probe RPM solution changed from green to red. Moreover, the probe has been successfully utilized for sensing HOCI in living cells.

• 出版日期2018-1
• 单位山东大学; 山东第一医科大学