Bone marrow Th17 TNF alpha cells induce osteoclast differentiation, and link bone destruction to IBD

作者：Ciucci Thomas; Ibanez Lidia; Boucoiran Agathe; Birgy Barelli Eleonore; Pene Jerome; Abou Ezzi Grazia; Arab Nadia; Rouleau Matthieu; Hebuterne Xavier; Yssel Hans; Blin Wakkach Claudine; Wakkach Abdelilah^*

来源：Gut, 2015, 64(7): 1072-1081.

DOI：10.1136/gutjnl-2014-306947

Objective Under both physiological and pathological conditions, bone volume is determined by the rate of bone formation by osteoblasts and bone resorption by osteoclasts. Excessive bone loss is a common complication of human IBD whose mechanisms are not yet completely understood. Despite the role of activated CD4(+) T cells in inflammatory bone loss, the nature of the T cell subsets involved in this process in vivo remains unknown. The aim of the present study was to identify the CD4(+) T cell subsets involved in the process of osteoclastogenesis in vivo, as well as their mechanism of action.
Design CD4(+) T cells were studied in IL10-/- mice and Rag1-/- mice adoptively transferred with naive CD4(+) CD45RB(high) T cells, representing two well-characterised animal models of IBD and in patients with Crohn's disease. They were phenotypically and functionally characterised by flow cytometric and gene expression analysis, as well as in in vitro cocultures with osteoclast precursors.
Results In mice, we identified bone marrow (BM) CD4(+) T cells producing interleukin (IL)-17 and tumour necrosis factor (TNF)-alpha as an osteoclastogenic T cell subset referred to as Th17 TNF-alpha(+) cells. During chronic inflammation, these cells migrate to the BM where they survive in an IL-7-dependent manner and where they promote the recruitment of inflammatory monocytes, the main osteoclast progenitors. A population equivalent to the Th17 TNF-alpha(+) cells was also detected in patients with Crohn's disease.
Conclusions Our results highlight the osteoclastogenic function of the Th17 TNF-alpha(+) cells that contribute to bone loss in vivo in IBD.

• 出版日期2015-7