mu-Crystallin, New Candidate Protein in Endotoxin-Induced Uveitis

作者：Imai Hiroki; Ohta Kouichi^*; Yoshida Akiko; Suzuki Satoru; Hashizume Kiyoshi; Usami Shinichi; Kikuchi Takanobu

来源：Investigative Ophthalmology & Visual Science, 2010, 51(7): 3554-3559.

DOI：10.1167/iovs.09-3728

PURPOSE. mu-Crystallin (CRYM) is a major taxon-specific lens protein. The purpose of this study was to investigate the function of CRYM in eyes of mice with endotoxin-induced uveitis (EIU).
METHODS. EIU was induced by an injection of a lipopolysaccharide (LPS) into the footpad of male C57BL/6J, CRYM knockout (CRYM(-/-)), and wild-type (CRYM(+/+)) mice. The expression of CRYM in the iris-ciliary body (ICB) was investigated by Western blot analyses and real-time RT-PCR at 12 hours and 1, 3, and 5 days after the LPS injection. The number of cells that had infiltrated the anterior chamber (AC) of the CRYM(+/+) mice was compared to that in the CRYM(-/-) mice at 1, 3, 5, and 7 days. The expressions of the mRNA of interleukin (IL)-1 alpha, IL-6, tumor necrosis factor (TNF)-alpha, and granulocyte macrophage- colony stimulating factor (GM-CSF) in the ICB of the two groups of mice were compared.
RESULTS. The mRNA of CRYM was upregulated at 12 hours after LPS injection, and CRYM protein increased at 3 days. The number of inflammatory cells in the AC of the CRYM(-/-) mice was not significantly different on day 1 from that in the CRYM(+/+) mice, but was significantly lower (17.9 +/- 1.6 vs. 27.1 +/- 2.4 cells/section) on day 5. Expression of the mRNA of IL-1 alpha and -6 in the CRYM(-/-) mice was significantly lower than that in the CRYM(+/+) mice on day 5.
CONCLUSIONS. CRYM plays an important role in the development of the second peak of murine EIU. (Invest Ophthalmol Vis Sci. 2010;51:3554-3559) DOI: 10.1167/iovs.09-3728

• 出版日期2010-7