Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples

作者：Hsia Chu Chieh^*; Chlzhikov Vladimir E; Yang Amy X; Selvapandiyan Angamuthu; Hewlett Indira; Duncan Robert; Puri Raj K; Nakhasi Hira L; Kaplan Gerardo G

来源：Biochemical and Biophysical Research Communications, 2007, 356(4): 1017-1023.

DOI：10.1016/j.bbrc.2007.03.087

• microarray technology
• human immunodeficiency virus type-1 (HIV-1)
• hepatitis C virus (HCV)
• hepatitis B virus (HBV)
• multiplex detection and discrimination
• PCR
• blood-borne virus

Hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type-1 (HIV-1) are transfusion-transmitted human pathogens that have a major impact oil blood safety and public health worldwide. We developed a microarray multiplex assay for the simultaneous detection and discrimination of these three viruses. The microarray consists of 16 oligonucleotide probes, immobilized on a silylated glass slide. Amplicons from multiplex PCR were labeled with Cy-5 and hybridized to the microarray. The assay detected 1 International Unit (IU), 10 IU, 20 IU of HBV, HCV, and HIV-1, respectively, in a single multiplex reaction. The assay also detected and discriminated the presence of two or three of these viruses in a single sample. Our data represent a proof-of-concept for the possible use of highly sensitive multiplex microarray assay to screen and confirm the presence of these viruses in blood donors and patients. Published by Elsevier Inc.

• 出版日期2007-5-18