AN EXPERIMENTAL STUDY OF THE POTENTIAL BIOLOGICAL EFFECTS ASSOCIATED WITH 2-D SHEAR WAVE ELASTOGRAPHY ON THE NEONATAL BRAIN

作者：Li, Changtian^*; Zhang, Changsheng; Li, Junlai; Cao, Xiaolin; Song, Danfei

来源：Ultrasound in Medicine and Biology, 2016, 42(7): 1551-1559.

DOI：10.1016/j.ultrasmedbio.2016.02.018

• 2-D Shear wave elastography
• Dynamic radiation force
• Bioeffects
• Brain development
• Learning
• Memory

2-D Shear wave elastography (SWE) imaging is widely used in clinical practice, and some researchers have applied this technique in the evaluation of neonatal brains. However, the immediate and long-term impacts of dynamic radiation force exposure on the neonatal central nervous system remain unknown. In this study, we exposed neonatal mice to 2-D SWE scanning for 10 min, 20 min and 30 min under diagnostic mode (mechanical index [MI]: 1.3; thermal index [TI]: 0.5), respectively. For the control group, the neonatal mice were sham irradiated for 30 min with the machine powered off. Their brains were collected and analyzed using histologic staining and western blot analysis at 24 h and 3 mo after the 2-D SWE scanning. The Morris water maze (MWM) test was used to assess learning and memory function of the mice at 3 mo of age. The results indicated that using 2-D SWEin evaluating brains of neonatal mice does not cause detectable histologic changes, nor does it have long-term effects on their learning and memory abilities. However, the PI3 K/AKT/mTOR pathway was disturbed when the 2-D SWE scanning lasted for more than 30 min, and the expression of p-PKCa was suppressed by 10 min or more in 2-D SWE scanning. Although these injuries may be self-repaired as the mice grow, more attention should be paid to the scanning duration when applying 2-D-SWE elastography in the assessment of neonatal brains. (E-mail: li_ jl@yeah.

• 出版日期2016-7
• 单位中国人民解放军总医院