Microbial Community Fingerprinting by Differential Display-Denaturing Gradient Gel Electrophoresis

作者:Portillo M C; Villahermosa D; Corzo A; Gonzalez J M*
来源:Applied and Environmental Microbiology, 2011, 77(1): 351-354.
DOI:10.1128/AEM.01316-10

摘要

Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison.

  • 出版日期2011-1