Activation Enhancement of Citric Acid Cycle to Promote Bioelectrocatalytic Activity of arcA Knockout Escherichia coli Toward High-Performance Microbial Fuel Cell

作者：Liu, Jing; Yong, Yang-Chun; Song, Hao^*; Li, Chang Ming

来源：ACS Catalysis, 2012, 2(8): 1749-1752.

DOI：10.1021/cs3003808

• microbial fuel cell
• bioelectrocatalysis
• citric acid cycle
• arcA knockout
• extracellular electron transfer
• glycerol

The bioelectrocatalysis in microbial fuel cells (MFCs) relies on both electrochemistry and metabolism of microbes. We discovered that under MFC microaerobic condition, an arcA knockout mutant Escherichia coli (arcA(-)) shows enhanced activation of the citric acid cycle (TCA cycle) for glycerol oxidation, as indicated by the increased key enzymes' activity in the TCA cycle. Meanwhile, a diffusive electron mediator (hydroxyl quinone derivative) is excreted by the genetically engineered arcA, resulting in a much higher power density than its parental strain toward glycerol oxidation. This work demonstrates that metabolic engineering is a feasible approach to construct efficient bioelectrocatalysts for high-performance MFCs.

• 出版日期2012-8
• 单位南阳理工学院; 江苏大学