No Involvement of Lysophosphatidic Acid Receptor-3 in Cell Migration of Mouse Lung Tumor Cells Stimulated by 12-O-Tetradecanoylphorbol-13-acetate

作者：Okumura Mai; Kato Kohei; Fukui Rie; Fukushima Nobuyuki; Tsujiuchi Toshifumi^*

来源：Journal of Toxicologic Pathology, 2011, 24(3): 183-186.

DOI：10.1293/tox.24.183

• LPA(3)
• 12-O-tetradecanoylphorbol-13-acetate
• cell migration
• lung
• mouse

The tumor promoting agent 12-O-tet radecanoylphorbol-13-acetate (TPA) stimulates cell migration of several tumor cells. Recently, we reported that loss of lysophosphatidic acid (LPA) receptor-3 (LPA(3)) enhanced cell migration of murine lung tumor LL/2 cells. In the present study, we investigated whether LPA3 is involved in cell migration of mouse lung tumor cells stimulated by TPA. Exogenous LPA3 gene (Lpar3)-expressing (LL/2-a3) cells and LL/2-AB cells as a vector control generated from LL/2 cells were used. In a cell migration assay, TPA treatment significantly stimulated cell migration of LL/2-AB and LL/2-a3 cells, while the cell migration abilities of LL/2-a3 were markedly lower than those of LL/2-AB cells. Using quantitative real-time reverse transcription (RT)-polymerase chain reaction (PCR) analysis, no effect of TPA treatment on the expression levels of LPA(1), LPA(2) and LPA(3) genes was detected in either type of cells. These results suggest that the LPA3 may not be involved in the enhanced migration ability by TPA in mouse lung tumor cells. (DOI: 10.1293/tox.24.183; J Toxicol Pathol 2011; 24: 183-186)

• 出版日期2011