A carbapenem-resistant Klebsiella pneumoniae epidemic clone in Jerusalem: sequence type 512 carrying a plasmid encoding aac(6)-Ib

作者：Warburg Gabriela; Hidalgo Grass Carlos; Partridge Sally R; Tolmasky Marcelo E; Temper Violeta; Moses Allon E; Block Colin; Strahilevitz Jacob^*

来源：Journal of Antimicrobial Chemotherapy, 2012, 67(4): 898-901.

DOI：10.1093/jac/dkr552

• Enterobacteriaceae
• carbapenemases
• aminoglycoside-modifying enzymes

We characterized distinctive features of a hypertransmissible carbapenem-resistant Klebsiella pneumoniae (CRKP) clone that emerged at Hadassah Hospital, Ein-Kerem, Jerusalem, Israel, in 2006.
Eleven CRKP isolated at Hadassah Hospital during 200509 were examined by antimicrobial susceptibility testing, PFGE and multilocus sequence typing (MLST). Plasmids were analysed by conjugation, restriction mapping, PCR and sequencing.
Divergence from the national epidemic sequence type (ST) ST258 to ST512 was observed early on. Carbapenem resistance was conferred by bla(KPC-3) carried on a plasmid apparently closely related to pKpQIL, also from Israel. This clone also carried a 15 kb plasmid, designated pAAC154, that carries a Tn1331 derivative containing the aac(6)-Ib gene. pAAC154 does not carry a bla(KPC) gene, but is similar to pS15, a plasmid from New York that carries bla(KPC-2).
A single CRKP clone ST512 has spread efficiently in our region. In this clone, aac(6)-Ib, common in CRKP strains, is carried on a different plasmid from bla(KPC-3).

• 出版日期2012-4