A mild TCEP-based para-azidobenzyl cleavage strategy to transform reversible cysteine thiol labelling reagents into irreversible conjugates

作者:Maruani Antoine; Alom Shamim; Canavelli Pierre; Lee Maximillian T W; Morgan Rachel E; Chudasama Vijay*; Caddick Stephen
来源:Chemical communications, 2015, 51(25): 5279-5282.
DOI:10.1039/c4cc08515a

摘要

It has recently emerged that the succinimide linkage of a maleimide thiol addition product is fragile, which is a major issue in fields where thiol functionalisation needs to be robust. Herein we deliver a strategy that generates selective cysteine thiol labelling reagents, which are stable to hydrolysis and thiol exchange.

  • 出版日期2015