Anaerobic ammonium-oxidizing bacteria gain antibiotic resistance during long-term acclimatization

作者：Zhang, Zheng-Zhe; Zhang, Qian-Qian; Guo, Qiong; Chen, Qian-Qian; Jiang, Xiao-Yan; Jin, Ren-Cun^*

来源：Bioresource Technology, 2015, 192: 756-764.

DOI：10.1016/j.biortech.2015.06.044

• Anammox
• Antibiotic resistance
• Inhibition
• Extracellular polymeric substances
• Acclimatization

Three broad-spectrum antibiotics, amoxicillin (AMX), florfenicol (FF) and sulfamethazine (SMZ), that inhibit bacteria via different target sites, were selected to evaluate the acute toxicity and long-term effects on anaerobic ammonium oxidation (anammox) granules. The specific anammox activity (SAA) levels reduced by approximately half within the first 3 days in the presence of antibiotics but no nitrite accumulation was observed in continuous-flow experiments. However, the SAA levels and heme c content gradually recovered as the antibiotic concentrations increased. Extracellular polymeric substances (EPS) analysis suggested that anaerobic ammonium-oxidizing bacteria gradually developed a better survival strategy during long-term acclimatization, which reduced the antibiotic stress via increased EPS secretion that provided a protective 'cocoon.' In terms of nitrogen removal efficiency, anammox granules could resist 60 mg-AMX L-1, 10 mg-FF L-1 and 100 mg-SMZ L-1. This study supported the feasibility of using anammox granules to treat antibiotic-containing wastewater.

• 出版日期2015-9
• 单位杭州师范大学