Architecture of the fungal nuclear pore inner ring complex

作者：Stuwe Tobias; Bley Christopher J; Thierbach Karsten; Petrovic Stefan; Schilbach Sandra; Mayo Daniel J; Perriches Thibaud; Rundlet Emily J; Jeon Young E; Collins Leslie N; Huber Ferdinand M; Lin Daniel H; Paduch Marcin; Koide Akiko; Lu Vincent; Fischer Jessica; Hurt Ed; Koide Shohei; Kossiakoff Anthony A; Hoelz Andre^*

来源：Science, 2015, 350(6256): 56-64.

DOI：10.1126/science.aac9176

The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. We present the reconstitution and interdisciplinary analyses of the similar to 425-kilodalton inner ring complex (IRC), which forms the central transport channel and diffusion barrier of the NPC, revealing its interaction network and equimolar stoichiometry. The Nsp1.Nup49.Nup57 channel nucleoporin heterotrimer (CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96. The CNT.Nic96 structure reveals that Nic96 functions as an assembly sensor that recognizes the three-dimensional architecture of the CNT, thereby mediating the incorporation of a defined CNT state into the NPC. We propose that the IRC adopts a relatively rigid scaffold that recruits the CNT to primarily form the diffusion barrier of the NPC, rather than enabling channel dilation.

• 出版日期2015-10-2