科研之友
微信
新浪微博
Facebook
分享链接
摘要
A simple method was developed for simultaneous determination of five amino acid neurotransmitters (taurine, glutamate, aspartate, glycine, and gamma-aminobutyric acid) in brain microdialysates by high-performance liquid chromatography with coulometric detection. The microdialysates were derivatized with o-phthalaldehyde in 5mM sodium sulfite and separated within 17min using isocratic elution. Detection parameters for these transmitters were linearity (r(2))>0.999 over the concentration range from 0.1 to 15.00 mu M (0.01-1.00 mu M for GABA); reproducibility 2.88%-11.67% (relative standard deviation); the limits of detection 4.4-17.2 fmol; and recovery 86.66%-103.42%. This method was successfully applied to analyze these neurotransmitters in the brain. The changes of neurotransmitters were also investigated via local administration of potassium chloride and bicuculline, which was a gamma-aminobutyric acid receptor antagonist. It was found that the developed method is more efficient as compared to previously reported methods in simultaneously determining five major neurotransmitters because it shows quick separation and sensitive detection, and is easy to operate. This method can be a practical choice for continuous monitoring of the amino acid neurotransmittersin vivo.
- 出版日期2015-9-14
- 单位中国科学院武汉物理与数学研究所; 湖北第二师范学院; 波谱与原子分子物理国家重点实验室; 中国科学院大学
