Anti-inflammatory effects of isoacteoside from Abeliophyllum distichum

作者：Nam Sun Young; Kim Hee Yun; Yoou Myoung Schook; Kim A Hyun; Park Byoung Jun; Jeong Hyun Ja^*; Kim Hyung Min

来源：Immunopharmacology and Immunotoxicology, 2015, 37(3): 258-264.

DOI：10.3109/08923973.2015.1026604

• Caspase-1
• isoacteoside
• MAPKs
• mast cells
• NF-kappa B

Isoacteoside, a dihydroxypheynylethyl glycoside, is a major bioactive component of Abeliophyllum distichum (White Forsythia) which is a deciduous shrub native to the south and central areas of Korea. The present study is designed to evaluate the anti-inflammatory activities and underlying mechanisms of isoacteoside in human mast cell line, HMC-1 cells. We isolated isoacteoside from A. distichum. The anti-inflammatory effect of isoacteoside was investigated in HMC-1 cells by studying the following markers: phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced interleukin (IL)-1 beta, IL-6, IL-8, and tumor necrosis factor alpha (TNF-alpha) secretion and mRNA expression by ELISA and RT-PCR, respectively. In addition, mechanism related to anti-inflammatory was investigated by Western blotting. Isoacteoside significantly suppressed the production and mRNA expression of proinflammatory cytokines including IL-1 beta, IL-6, IL-8 and TNF-alpha in PMACI-stimulated HMC-1 cells without cytotoxicity. It was found that anti-inflammatory effects of isoacteoside are mediated by action on caspase-1, mitogen-activated protein kinases (c-Jun N-terminal kinase, p38, extracellular signal-regulated protein kinase) and nuclear factor-kappa B pathways. Taken together, the present findings provide new insights that isoacteoside may be a promising anti-inflammatory agent for inflammatory disorders.

• 出版日期2015-6