A novel method for the measurement of plasma metanephrines using online solid phase extraction-liquid chromatography tandem mass spectrometry

作者：Adaway Joanne E^*; Peitzsch Mirko; Keevil Brian G

来源：Annals of Clinical Biochemistry, 2015, 52(3): 361-369.

DOI：10.1177/0004563214546560

• Metanephrine
• normetanephrine
• 3-methoxytyramine
• phaeochromocytoma
• LC-MS
• MS
• online solid phase extraction

Background Measurement of plasma metanephrine, normetanephrine and 3-methoxytyramine is useful in the diagnosis of phaeochromocytomas, but many assays require a large volume of plasma due to poor assay sensitivity, and often require lengthy sample preparation. Our aim was to develop a method for measurement of plasma metanephrines using a small sample volume with minimal hands-on preparation.
Methods Samples were deproteinised using 10K spin filters prior to online solid phase extraction using a Waters Acquity UPLC Online SPE Manager (Waters, Manchester, UK) coupled to a Waters Xevo TQ-S mass spectrometer (Waters, Manchester, UK). The assay was validated and results compared to a previously published method.
Results We achieved a limit of quantification of 37.5pmol/L for metanephrine and 3-methoxytyramine and 75pmol/L for normetanephrine using only 150 mu L of sample. The assay was linear up to 30,000pmol/L for all analytes and in a method comparison study results showed good agreement with a previously published LC-MS/MS assay.
Conclusions We have developed a simple method for measurement of plasma metanephrine, normetanephrine and 3-methoxytyramine using only 150 mu L of sample. There is minimal hands-on sample preparation required and the assay is suitable for routine use in a clinical laboratory.

• 出版日期2015-5