A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)

作者：Phan Tung G; Dreno Brigitte; da Costa Antonio Charlys; Li Linlin; Orlandi Patricia; Deng Xutao; Kapusinszky Beatrix; Siqueira Juliana; Knol Anne Chantal; Halary Franck; Dantal Jacques; Alexander Kathleen A; Pesavento Patricia A; Delwart Eric

来源：Virology, 2016, 496: 299-305.

DOI：10.1016/j.virol.2016.06.013

• Cutavirus
• Protoparvovirus
• Oncolytic
• CTCL

We genetically characterized seven nearly complete genomes in the protoparvovirus genus from the feces of children with diarrhea. The viruses, provisionally named cutaviruses (CutaV), varied by 1-6% nucleotides and shared similar to 76% and similar to 82% amino acid identity with the NS1 and VP1 of human bufaviruses, their closest relatives. Using PCR, cutavirus DNA was found in 1.6% (4/245) and 1% (1/100) of diarrhea samples from Brazil and Botswana respectively. In silico analysis of pre-existing metagenomics datasets then revealed closely related parvovirus genomes in skin biopsies from patients with epidermotropic cutaneous T-cell lymphoma (CTCL or mycosis fungoides). PCR of skin biopsies yielded cutavirus DNA in 4/17 CTCL, 0/10 skin carcinoma, and 0/21 normal or noncancerous skin biopsies. In situ hybridization of CTCL skin biopsies detected viral genome within rare individual cells in regions of neoplastic infiltrations. The influence of cutavirus infection on human enteric functions and possible oncolytic role in CTCL progression remain to be determined.

• 出版日期2016-9
• 单位Virginia Tech; 美国弗吉尼亚理工大学(Virginia Tech)