Display of Clostridium cellulovorans Xylose Isomerase on the Cell Surface of Saccharomyces cerevisiae and its Direct Application to Xylose Fermentation

作者：Ota Miki; Sakuragi Hiroshi; Morisaka Hironobu; Kuroda Kouichi; Miyake Hideo; Tamaru Yutaka; Ueda Mitsuyoshi^*

来源：Biotechnology Progress, 2013, 29(2): 346-351.

DOI：10.1002/btpr.1700

• xylose isomerase
• Clostridium cellulovorans
• Saccharomyces cerevisiae
• cell surface engineering

Xylose isomerase (XI) is a key enzyme in the conversion of d-xylose, which is a major component of lignocellulosic biomass, to d-xylulose. Genomic analysis of the bacterium Clostridium cellulovorans revealed the presence of XI-related genes. In this study, XI derived from C. cellulovorans was produced and displayed using the yeast cell-surface display system, and the xylose assimilation and fermentation properties of this XI-displaying yeast were examined. XI-displaying yeast grew well in medium containing xylose as the sole carbon source and directly produced ethanol from xylose under anaerobic conditions.

• 出版日期2013-4