The clinical and phylogenetic investigation for a nosocomial outbreak of respiratory syncytial virus infection in an adult hemato-oncology unit

作者：Nabeya Daijiro; Kinjo Takeshi; Parrott Gretchen Lynn; Uehara Ayako; Motooka Daisuke; Nakamura Shota; Nahar Saifun; Nakachi Sawako; Nakamatsu Masashi; Maeshiro Sakuko; Haranaga Shusaku; Tateyama Masao; Tomoyose Takeaki; Masuzaki Hiroaki; Horii Toshihiro; Fujita Jiro

来源：Journal of Medical Virology, 2017, 89(8): 1364-1372.

DOI：10.1002/jmv.24800

• hemato-oncology patient
• outbreak
• phylogenetic analysis
• rapid antigen test
• respiratory syncytial virus
• viral shedding

Although many reports have already shown RSV outbreaks among hemato-oncology patients, genomic studies detecting similar RSV strains prior to an outbreak in the hospital are rare. In 2014, the University of the Ryukyus hospital hemato-oncology unit experienced, and successfully managed, a respiratory syncytial virus (RSV) nosocomial outbreak. During the outbreak investigation, genotyping and phylogenetic analysis was used to identify a potential source for the outbreak. Nasopharyngeal swabs were tested for RSV using three tests: (1) rapid antigen test (RAT); (2) reverse transcriptase polymerase chain reaction (PCR); or (3) quantitative PCR (RT-qPCR); a positive PCR reaction was considered a confirmed case of RSV. Phylogenetic analysis of theGprotein was performed for outbreak and reference samples from non-outbreak periods of the same year. In total, 12 confirmed cases were identified, including 8 hematooncology patients. Patient samples were collected weekly, until all confirmed RSV cases returned RSV negative test results. Median time of suspected viral shedding was 16 days (n = 5, range: 8-37 days). Sensitivity and specificity of the RAT compared with RT-qPCR were 30% and 91% (n = 42). Phylogenetic analysis revealed nine genetically identical strains; eight occurring during the outbreak time period and one strain was detected 1 month prior. A genetically similar RSV detected 1 month before is considered one potential source of this outbreak. As such, healthcare providers should always enforce standard precautions, especially in the hematooncology unit.

• 出版日期2017-8