Selective Phosphonylation of 5 '-Adenosine Monophosphate (5 '-AMP) via Pyrophosphite [PPi(III)]

作者：Kaye Karl; Bryant David E; Marriott Katie E R; Ohara Shohei; Fishwick Colin W G; Kee Terence P^*

来源：Origins of Life and Evolution of the Biosphere, 2016, 46(4): 425-434.

DOI：10.1007/s11084-016-9497-y

• Phosphorus
• Prebiotic Chemistry
• Origin of Life
• Nucleotides

We describe here experiments which demonstrate the selective phospho-transfer from a plausibly prebiotic condensed phosphorus (P) salt, pyrophosphite [H2P2O5 (2-); PPi(III)], to the phosphate group of 5'-adenosine mono phosphate (5'-AMP). We show further that this P-transfer process is accelerated both by divalent metal ions (M2+) and by organic co-factors such as acetate (AcO-). In this specific case of P-transfer from PPi(III) to 5'-AMP, we show a synergistic enhancement of transfer in the combined presence of M2+ & AcO-. Isotopic labelling studies demonstrate that hydrolysis of the phosphonylated 5'-AMP, [P(III)P(V)-5'-AMP], proceeds via nuceophilic attack of water at the Pi(III) terminus.

• 出版日期2016-12