Macrophages Induce Long-Term Trapping of gamma delta T Cells with Innate-like Properties within Secondary Lymphoid Organs in the Steady State

作者：Audemard Verger Alexandra; Riviere Matthieu; Durand Aurelie; Peranzoni Elisa; Guichard Vincent; Hamon Pauline; Bonilla Nelly; Guilbert Thomas; Boissonnas Alexandre; Auffray Cedric; Eberl Gerard; Lucas Bruno; Martin Bruno^*

来源：The Journal of Immunology, 2017, 199(6): 1998-2007.

DOI：10.4049/jimmunol.1700430

So far, peripheral T cells have mostly been described to circulate between blood, secondary lymphoid organs (SLOs), and lymph in the steady state. This nomadic existence would allow them to accomplish their surveying task for both foreign Ags and survival signals. Although it is now well established that gamma delta T cells can be rapidly recruited to inflammatory sites or in certain tumor microenvironments, the trafficking properties of peripheral gamma delta T cells have been poorly studied in the steady state. In the present study, we highlight the existence of resident gamma delta T cells in the SLOs of specific pathogen-free mice. Indeed, using several experimental approaches such as the injection of integrin-neutralizing Abs that inhibit the entry of circulating lymphocytes into lymph nodes and long-term parabiosis experiments, we have found that, contrary to Ly-6C(-/+)CD44(lo) and Ly-6C(+)CD44(hi) gamma delta T cells, a significant proportion of Ly-6C(-)CD44(hi) gamma delta T cells are trapped for long periods of time within lymph nodes and the spleen in the steady state. Specific in vivo cell depletion strategies have allowed us to demonstrate that macrophages are the main actors involved in this long-term retention of Ly-6C(-)CD44(hi) gamma delta T cells in SLOs.

• 出版日期2017-9-15