Addition of 2-Deoxyglucose Enhances Growth Inhibition But Reverses Acidification in Colon Cancer Cells Treated with Phenformin

作者：Lea Michael A^*; Chacko Jerel; Bolikal Sandhya; Hong Ji Y; Chung Ryan; Ortega Andres; desBordes Charles

来源：Anticancer Research, 2011, 31(2): 421-426.

• Colon cancer
• metformin
• phenformin
• 2-deoxyglucose
• acidification

A report that effects of butyrate on some cells may be mediated by activation of AMP-activated protein kinase (AMPK) prompted this study which examines if other AMPK activators can induce differentiation and inhibit proliferation of colon cancer cells in a manner similar to butyrate. Using induction of alkaline phosphatase as a marker, it was observed that compound C, an AMPK inhibitor, is able to reduce the differentiating effect of butyrate on SW1116 and Caco-2 colon cancer cells. Metformin was observed to be less effective than butyrate in the induction of alkaline phosphatase but was more effective as a growth inhibitor. Phenformin was found to be a more potent growth inhibitor than metformin and both compounds cause acidification of the medium when incubated with colon cancer cells. Combined incubation of 2-deoxyglucose with either of the biguanides prevented the acidification of the medium but enhanced the growth inhibitory effects.

• 出版日期2011-2