Antioxidant Activity and Determination of Phenolic Compounds from Eugenia involucrata DC. Fruits by UHPLC-MS/MS

作者：Nicacio Antonio Eduardo; Rotta Eliza Mariane; Boeing Joana Schuelter; Barizao Erica Oliveira; Kimura Elza; Visentainer Jesui Vergilio; Maldaner Liane

来源：Food Analytical Methods, 2017, 10(8): 2718-2728.

DOI：10.1007/s12161-017-0840-3

• Eugenia involucrata DC.
• Phenolic compounds
• Antioxidant activity
• UHPLC-MS/MS

Fruits have been the focus of several studies aimed at finding new antioxidant sources for protection against the damage caused by reactive species. In this study, the antioxidant activity and the presence of phenolic compounds in all parts (peel, pulp, and seeds) of Eugenia involucrata DC. fruits were evaluated. DPPH center dot, ABTS(center dot+), and ORAC methods were used to determine the antioxidant activity, and an UHPLC-MS/MS method was developed for determining the phenolic compounds (gallic, chlorogenic, ferulic, p-coumaric and ellagic acids, quercetin, and myricetin). In the determination of both antioxidant activity and phenolic composition, the efficiency of solvents with different polarities-methanol/H2O (80:20, v/v), ethanol/H2O (80:20, v/v), methanol/acidified water with phosphoric acid pH 3.00 (80:20, v/v), and ethyl acetate-for the extraction of the phenolic compounds, was also evaluated. All parts of E. involucrata fruits showed antioxidant activity, in the range of 36.68 +/- 1.44 to 873.87 +/- 18.24 mu mol TE g(-1), being the highest values found in the seeds and peel when more polar extraction solvents were used. Six, five, and three phenolic compounds were identified and quantified in the pulp, peel, and seeds, respectively, with the highest abundance as p-coumaric acid (14 +/- 2 mg kg(-1)) in the pulp, quercetin (47 +/- 5 mg kg(-1)) in the peel, and gallic acid (74 +/- 4 mg kg(-1)) in the seeds, also when more polar solvents were used. Although antioxidant activity methods suggested that the peel and seeds have more antioxidant potential, a wider variety of compounds were determined in the pulp.

• 出版日期2017-8