A recombinant flagellin fragment, which includes the epitopes flg22 and flgII-28, provides a useful tool to study flagellin-triggered immunity

作者：Ciarroni Serena; Clarke Christopher R; Liu Haijie; Eckshtain Levi Noam; Mazzaglia Angelo; Balestra Giorgio M; Vinatzer Boris A^*

来源：Journal of General Plant Pathology, 2018, 84(3): 169-175.

DOI：10.1007/s10327-018-0779-2

• Flagellin
• FliC
• Kiwifruit
• Reactive oxygen species
• PAMP

Plants and animals independently evolved the ability to recognize flagellin (also called FliC), the building block of the bacterial flagellum, as part of their innate immune response. While animals recognize a relatively large region of FliC, most plants recognize one or two short epitopes of FliC: flg22 and flgII-28. However, since most research in plants has focused on flg22 and flgII-28 and not the actual FliC protein, the importance of any FliC region beyond the two epitopes in plant immunity is poorly understood. Here we report cloning, overexpression, and purification of a Pseudomonas syringae FliC fragment from amino acid 1 to 143, which includes both FliC epitopes and the adjacent alpha helices. Exposing Arabidopsis thaliana leaves to FliC(1-143) did not reveal any additional FliC recognition capabilities beyond flg22. However, while the kiwifruit species Actinidia arguta did not respond to either flg22 or flgII-28, treatment of A. arguta leaves with FliC(1-143) triggered a significant reactive oxygen response, indicating recognition. This result suggests that in some plant species, recognition of FliC requires regions of FliC beyond the two well-known epitopes and that FliC(1-143) represents a useful tool in the study of plant immunity.

• 出版日期2018-5
• 单位美国弗吉尼亚理工大学(Virginia Tech)