A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control

作者：Kume Kazunori^*; Cantwell Helena; Neumann Frank R; Jones Andrew W; Snijders Ambrosius P; Nurse Paul

来源：PLoS Genetics, 2017, 13(5): e1006767.

DOI：10.1371/journal.pgen.1006767

How cells control the overall size and growth of membrane-bound organelles is an important unanswered question of cell biology. Fission yeast cells maintain a nuclear size proportional to cellular size, resulting in a constant ratio between nuclear and cellular volumes (N/C ratio). We have conducted a genome-wide visual screen of a fission yeast gene deletion collection for viable mutants altered in their N/C ratio, and have found that defects in both nucleocytoplasmic mRNA transport and lipid synthesis alter the N/C ratio. Perturbing nuclear mRNA export results in accumulation of both mRNA and protein within the nucleus, and leads to an increase in the N/C ratio which is dependent on new membrane synthesis. Disruption of lipid synthesis dysregulates nuclear membrane growth and results in an enlarged N/C ratio. We propose that both properly regulated nucleocytoplasmic transport and nuclear membrane growth are central to the control of nuclear growth and size.

• 出版日期2017-5