A Simple Electrochemical Aptamer Cytosensor for Direct Detection of Chronic Myelogenous Leukemia K562 Cells

作者：Yu, Tianxiao; Zhang, Hui; Huang, Zhenglan; Luo, Zhenhong; Huang, Ningshu; Ding, Shijia^*; Feng, Wenli^*

来源：Electroanalysis, 2017, 29(3): 828-834.

DOI：10.1002/elan.201600505

• Electrochemical aptamer cytosensor
• chronic myelogenous leukemia
• K562 cell

A simple and rapid electrochemical aptamer cytosensor has been developed for direct detection of chronic myelogenous leukemia (CML) K562 cells based on a specific aptamer and a biotin conjugated concanavalin A (bio-ConA) detection probe. The K562 cell could be specifically recognized by T2-KK1B10 capture aptamer pre-immobilized on gold modified electrode surface. Then, bio-ConA was added in the reaction to identify K562 cell surface mannose, resulting in an aptamer-K562 cell-bio-ConA sandwich complex. Finally, streptavidin conjugated alkaline phosphatase (ST-ALP) combined with the bio-ConA to catalyze -naphthyl (-NP) phosphate to form -naphthol which is highly electroactive at an operating voltage of 180mV (vs. Ag/AgCl). Under optimum conditions, the DPV signals were proportional to the logarithm of K562 cell from 1x10(2) to 1x10(7)cellsmL(-1) with a detection limit of 79cellsmL(-1). The cytosensor also exhibited high selectivity, stability and reproducibility. When applied to detect K562 cells in human blood samples, recoveries between 79.6%-93.3% were obtained, indicating the developed biosensor would be a potential alternative tool for CML K562 cell detection in real biological samples.

• 出版日期2017-3
• 单位重庆医科大学