科研之友
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摘要
Seven kinds of alpha-globin genes were isolated from the genomic library of carp Cyprinus carpio chromosomal DNA. In this study, the complete nucleotide sequences of three carp alpha-globin genes (Nos. 3, 6, and 7) were determined. These carp genes had analogue with the arrangement of translated, untranslated, and intervening sequences of the vertebrate alpha-globin gene, i.e. two intervening sequences interrupting the coding region into three parts. The coding region of exon 1, 2, and 3 in Nos. 3, 6, and 7 were 95 base pair (bp), 208 bp, and 129 bp, respectively. The first and second intervening sequences of No. 3 were 159 bp and 96 bp in length, respectively. The intervening sequences of No. 6 were 179 bp and 99 bp, and of No. 7 159 bp and 92 bp in length, respectively. In the 5'- and 3'-untranslated region, sequences homologous with the consensus sequence of the signals for expression were observed. The individuality of the nucleotide sequences suggests that the carp alpha-globin genes constitute a family structure. The amino acid residues of the predicted carp alpha-globin concern quaternary structure were extremely conserved with other vertebrate, e.g., human, duck, and frog.
- 出版日期1993-6
