科研之友
微信
新浪微博
Facebook
分享链接
摘要
Objective: We detected immunohistochemistry location of follicle-stimulating hormone (FSH) and its receptor (FSHR), and further studied its gene sequence characteristics, providing morphological basis for studying FSH function in regulating liver metabolic function. Methods: FSH and FSHR localization were analyzed by double-label immunofluorescence method. The levels of FSH and FSH mRNA in rat liver cells were detected by RT-PCR, and the obtained cDNA was used for gene cloning and sequence analysis of the core sequence. Results: FSH and FSHR were expressed in cytoplasm of rat liver cells with different density and degree of immunostaining in liver cells in different parts. Green fluorescence appeared in cytoplasm of FSH positive cells, and red fluorescence appeared in cytoplasm of FSHR positive cells. Some liver cells expressed both FSH and FSHR, showing red and yellow fluorescence. FSH and FSHR gene in liver cells expressed similarly, as revealed by mRNA analysis. Sequence analysis was performed on the specific bands of FSH and FSHR gene amplified from rat liver tissue, and the results suggested that in the amplified products, FSH gene sequence was consistent with genes in rat adenohypophysis; FSHR gene sequence was consistent with rat testis, which was previously reported. Conclusion: FSH and FSHR were expressed in a portion of rat liver cells. FSHR expression level was different in liver cells in different parts. FSH and FSHR only coexisted in a portion of liver cells. FSH may regulate liver functions through autocrine or paracrine.
- 出版日期2016
- 单位德州学院
