Molecular characterization of the HIV type 1 vpr gene in infected chinese former blood/plasma donors at different stages of diseases

作者：Shen, Chengli; Gupta, Phalguni; Wu, Hao; Chen, Xinyue; Huang, Xiaojie; Zhou, Yusen; Chen, Yue^*

来源：AIDS Research and Human Retroviruses, 2008, 24(4): 661-666.

DOI：10.1089/aid.2007.0270

• IMMUNODEFICIENCY-VIRUS TYPE-1
• VIRAL PROTEIN-R
• REVERSE TRANSCRIPTION COMPLEXES
• NUCLEAR-PORE COMPLEX
• CELL-CYCLE ARREST
• TISSUE EX-VIVO
• HIV-1 VPR
• FUNCTIONAL DOMAINS
• R77Q MUTATION
• BLOOD-DONORS

The HIV-1 vpr regions were PCR amplified and sequenced from eight long-term nonprogressors' (LTNP) and seven AIDS patients' DNA samples of a cohort of HIV-1- infected Chinese plasma/blood donors (PBDs). Sequence analysis revealed that the patients' HIV-1 vpr sequences belong to HIV-1 subtype B and there were no differences in the divergence of vpr sequences between these two groups of patients. Similarly, in the deduced amino acid sequences, no significant differences have been detected in vpr functional domains from patients at different stages of the disease. Moreover, the predicted binding motifs of HLA A2 and A11 were highly conserved among patients' vpr amino acid sequences. These results show that vpr may not play an important role in HIV-1 pathogenesis in different stages of Chinese patients and may have important implications in developing vpr- related treatments suitable for HIV-1-infected PBDs.

• 出版日期2008-4
• 单位首都医科大学