A spinosyn-sensitive Drosophila melanogaster nicotinic acetylcholine receptor identified through chemically induced target site resistance, resistance gene identification, and heterologous expression

作者：Watson Gerald B^*; Chouinard Scott W; Cook Kevin R; Geng Chaoxian; Gifford Jim M; Gustafson Gary D; Hasler James M; Larrinua Ignacio M; Letherer Ted J; Mitchell Jon C; Pak William L; Salgado Vincent L; Sparks Thomas C; Stilwell Geoff E

来源：Insect Biochemistry and Molecular Biology, 2010, 40(5): 376-384.

DOI：10.1016/j.ibmb.2009.11.004

• Spinosad
• Spinetoram
• Spinosyn
• Resistance
• Drosophila melanogaster
• Nicotinic acetylcholine receptor (nAChR)
• Xeno pus laevis oocytes
• Expression
• Mode of action

Strains of Drosophila melanogaster with resistance to the insecticides spinosyn A, spinosad, and spinetoram were produced by chemical mutagenesis. These spinosyn-resistant strains were not cross-resistant to other insecticides. The two strains that were initially characterized were subsequently found to have mutations in the gene encoding the nicotinic acetylcholine receptor (nAChR) subunit D alpha 6 Subsequently, additional spinosyn-resistant alleles were generated by chemical mutagenesis and were also found to have mutations in the gene encoding D alpha 6, providing convincing evidence that D alpha 6 is a target site for the spinosyns in D. melanogaster. Although a spinosyn-sensitive receptor could not be generated in Xenopus laevis oocytes simply by expressing D alpha 6 alone, co-expression of D alpha 6 with an additional nAChR subunit, D alpha 5, and the chaperone protein ric-3 resulted in an acetylcholine- and spinosyn-sensitive receptor with the pharmacological properties anticipated for a native nAChR.

• 出版日期2010-5