Arabidopsis sensitivity to protein synthesis inhibitors depends on 26S proteasome activity

作者：Kurepa Jasmina; Karangwa Consolee; Duke Liliana Sfichi; Smalle Jan A^*

来源：Plant Cell Reports, 2010, 29(3): 249-259.

DOI：10.1007/s00299-010-0818-8

• Arabidopsis thaliana
• Cycloheximide
• Herbicide tolerance
• Hygromycin B
• L-Phosphinothricin
• Proteolysis

The 26S proteasome (26SP), the central protease of the ubiquitin-dependent proteolysis pathway, controls the regulated proteolysis of functional proteins and the removal of misfolded and damaged proteins. In Arabidopsis, cellular and stress response phenotypes of a number of mutants with partially impaired 26SP function have been reported. Here, we describe the responses of proteasome mutants to protein synthesis inhibitors. We show that the rpt2a-3, rpn10-1 and rpn12a-1 mutants are hypersensitive to the antibiotic hygromycin B, and tolerant to the translation inhibitor cycloheximide (CHX) and herbicide l-phosphinothricin (PPT). In addition to the novel mechanism for herbicide tolerance, our data suggests that the combination of hygromycin B, CHX and PPT growth-response assays could be used as a facile diagnostic tool to detect altered 26SP function in plant mutants and transgenic lines.

• 出版日期2010-3