摘要

A simple and rapid flotation method for separating insect fragments in bran and fine bran was developed. A 25 g sample is properly hydrated by immersion and stirring (10 min) in hot (90 degrees C) distilled water (600 mL) and then sieved (mesh sieve n. 140) under jets of cold tap water. The trapping is quantitatively transferred to a Wildman trap flask, dissolved in ethanol (60%) and floated with light mineral oil. The material floated is paper-filtered with a Buchner funnel under vacuum. Material obtained from three consecutive flotations and filtrations are microscopically examined and the insect fragments are counted. The new method which requires low input of facilities and non-toxic reagents could be useful to detect insect fragments in bran and fine bran used for the production of whole food or animal feed.

  • 出版日期2010