A new method for the determination of the relative affinity of a ligand against various DNA sequences by electrospray ionization mass spectrometry. Application to a polyamide minor groove binder

作者：Buchmann William^*; Boutorine Alexandre; Halby Ludovic; Tortajada Jeanine; De Pauw Edwin

来源：Journal of Mass Spectrometry, 2009, 44(8): 1171-1181.

DOI：10.1002/jms.1592

• mass spectrometry
• electrospray ionization
• noncovalent interactions
• DNA recognition
• polyamide
• minor groove binder

A new method for the determination of the relative affinity of a ligand against various dsDNA sequences is presented by using electrospray ionization time-of-flight mass spectrometry (ESI-QTOF) mass spectrometry. The principle is described here through the complexation of double-stranded DNA by a polyamide ligand including twelve N-methylpyrrole rings. However this method could be applied to other ligands especially when dissociation constants (K(d)) are in nanomolar range. This method does not require knowing the ligand concentration accurately. It allows determination of the relative affinity of a ligand against various dsDNA sequences for 1:1 complex stoichiometries in a quick manner without labeling.

• 出版日期2009-8
• 单位中国地震局