A rapid and highly sensitive protocol for the detection of Escherichia coli O157:H7 based on immunochromatography assay combined with the enrichment technique of immunomagnetic nanoparticles

作者：Qi, Hui; Zhong, Zhen; Zhou, Han-Xin; Deng, Chun-Yan; Zhu, Hai; Li, Jin-Feng; Wang, Xi-Li; Li, Fu-Rong^*

来源：International Journal of Nanomedicine, 2011, 6: 3033-3039.

DOI：10.2147/IJN.S25684

• colloidal gold
• immunomagnetic nanoparticles
• Escherichia coli O157:H7
• immunochromatographic assay

Background: Escherichia coli O157:H7 (E. coli O157: H7) is an important pathogenic bacterium that threatens human health. A rapid, simple, highly sensitive, and specific method for the detection of E. coli O157: H7 is necessary. @@@ Methods: In the present study, immunomagnetic nanoparticles (IMPs) were prepared with nanopure iron as the core, coated with E. coli O157: H7 polyclonal antibodies. These IMPs were used in combination with immunochromatographic assay (ICA) and used to establish highly sensitive and rapid kits (IMPs+ICA) to detect E. coli O157: H7. The kits were then used to detect E. coli O157: H7 in 150 food samples and were compared with conventional ICA to evaluate their efficacy. @@@ Results: The average diameter of IMPs was 56 nm and the amount of adsorbed antibodies was 106.0 mu g/mg. The sensitivity of ICA and IMPs+ICA was 10(5) colony-forming units/mL and 10(3) CFUs/mL, respectively, for purified E. coli O157:H7 solution. The sensitivity of IMPs+ICA was increased by two orders, and its specificity was similar to ICA. @@@ Conclusion: The kits have the potential to offer important social and economic benefits in the screening, monitoring, and control of food safety.

• 出版日期2011
• 单位暨南大学