Anti-Tumor Effect of Adenoviral Vector-Mediated p53 Gene Transfer on the Growth of Canine Osteosarcoma Xenografts in Nude Mice

作者：Kanaya Noriko; Yazawa Mitsuhiro; Goto Koshino Yuko; Mochizuki Manabu; Nishimura Ryohei; Ohno Koichi; Sasaki Nobuo; Tsujimoto Hajime^*

来源：Journal of Veterinary Medical Science, 2011, 73(7): 877-883.

DOI：10.1292/jvms.10-0570

• adenovirus vector
• canine
• mouse model
• osteosarcoma
• P53

We evaluated the anti-tumor effect of adenoviral vector-mediated p53 gene therapy on the growth of canine osteosarcoma xenografts formed in nude mice. Nude mice were subcutaneously transplanted with cells of 2 P53 mutant canine osteosarcoma cell lines, POS and CHOS. The osteosarcoma xenografts were injected with either an adenoviral vector that expresses canine wild-type P53 (AxCA-cp53) or LacZ (AxCA-LacZ). Tumor growth was significantly inhibited in the xenografts injected with AxCA-cp53 in comparison to those injected with AxCA-LacZ or PBS during the observation period of 27 days. An increase of the amount of p21(WAFI/CDKNIA) mRNA, and the number of apoptotic cells was shown in the tumors injected with AxCA-cp53 in comparison to those injected with AxCA-LacZ or PBS. The present study revealed that the adenoviral vector-mediated p53 gene transfer had an anti-tumor effect in canine osteosarcoma xenografts formed in nude mice.

• 出版日期2011-7