How to make DNA count: DNA-based diagnostic tools in veterinary parasitology

作者：Hunt P W^*; Lello J

来源：Veterinary Parasitology, 2012, 186(1-2): 101-108.

DOI：10.1016/j.vetpar.2011.11.055

• Diagnosis
• DNA methods
• Faecal egg count
• Sheep
• Goat
• Cattle

Traditional methods for the diagnosis of parasitic helminth infections of livestock have a number of limitations, such as the inability to distinguish mixed-species infections, a heavy reliance on technical experience and also sub-sampling errors. Some of these limitations may be overcome through the development of rapid and accurate DNA-based tests. For example, DNA-based tests can specifically detect individual species in a mixed infection at either the larval or egg stages, in the absence of morphological differences among species. Even so, some diagnostic problems remain the same, irrespective of whether a DNA-based or traditional method is used. For example, sub-sampling errors from an aggregated distribution are likely to persist. It is proposed, however, that DNA-based diagnostic technologies offer an opportunity to expand diagnostic capabilities, and are discussed in the current review. The future introduction of DNA-based diagnostic technologies into routine diagnostic settings will also be discussed.

• 出版日期2012-5-4
• 单位CSIRO