科研之友
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摘要
CD5 is a cell Surface molecule involved in antigen recognition and is present on all T lymphocytes and a subset of B lymphocytes. The purpose of this Study was to examine CD5+ expression on peripheral blood B cells from healthy, noninfected cattle and cattle with subclinical and clinical paratuberculosis. Peripheral blood mononuclear cells (PBMC) were freshly isolated or cultured for 7 days in the presence or absence of live Mycobacterium avium subsp. paratuberculosis (M. avium subsp. paratuberculosis), and then analyzed by flow cytometry for CD5 expression within the B cell subpopulation. Analysis demonstrated a significant increase (P < 0.01) in B cells in clinical animals as compared to healthy control cows and subclinically infected cows. In addition, three subpopulations within the CD5+ B cell population were identified: CD5(dim), CD5(bright), and a minor population that was characterized as CD5(extra) (bright). A decrease in the CD5(dim) B cell population along with a concomitant increase in CD5(bright) B cells was observed in infected cows, an effect that was highly significant (P < 0.01) for subclinically infected cows in Cultured PBMC. In vitro infection with live M. avium subsp. paratuberculosis did not affect CD5+ expression patterns on B cells, regardless of animal infection status. Addition of exogenous IL-10 to PBMC cultures resulted in decreased numbers of CD5(bright) B cells for healthy control cows, whereas, a synergistic effect of IL-10 and infection with live M. avium subsp. paratuberculosis resulted in increased CD5(bright) B cells for subclinically infected cows. These results suggest that differential expression of CD5(bright) and CD5(dim) subpopulations on B cells in animals with paratuberculosis may reflect a shift in host immunity during the disease process. Published by Elsevier B.V.
- 出版日期2008-12-15
