A nanoprobe for nonprotein thiols based on assembling of QDs and 4-amino-2,2,6,6-tetramethylpiperidine oxide

作者：Xu, Kehua; Chen, Huachao; Wang, Huixia; Tian, Jiangwei; Li, Jing; Li, Qingling; Li, Na; Tang, Bo^*

来源：Biosensors and Bioelectronics, 2011, 26(11): 4632-4636.

DOI：10.1016/j.bios.2011.05.020

• Fluorescent probe
• Nonprotein thiol
• Quantum dots
• Cell imaging

A new fluorescent nanoprobe. 4-amino-2,2,6,6-tetramethylpiperidine oxide (AT)-functionalized CdTe quantum dots (QDs-AT), was synthesized, for selective detection of nonprotein thiols based on electron transfer (ET). In the presence of nonprotein thiols, the nitroxide radicals in QDs-AT were converted to hydroxylamines, resulting in the fluorescence recovery of the quenched QDs. The detection mechanism of the probe was investigated using Rh-Se-2 probe. The nanoprobe has high sensitivity toward glutathione (GSH) with a detection limit of 7.1 x 10(-8) M. The fluorescent imaging of living cells showed that QDs-AT could distinguish the concentration differences of GSH in HL-7702 and HepG2 cells.

• 出版日期2011-7-15
• 单位山东师范大学