Molecular Mechanisms Underlying the Time-dependent Autophagy and Apoptosis Induced by Nutrient Depletion in Multiple Myeloma: a Pilot Study

作者：Liu Yuan; Chen Yan; Wen Lu; Cui Guohui^*

来源：Journal of Huazhong University of Science and Technology-Medical Sciences, 2012, 32(1): 1-8.

DOI：10.1007/s11596-012-0001-2

• multiple myeloma
• RPMI8226
• autophagy
• apoptosis
• Deptor
• Raf-1/JNK/p53/p21
• NF kappa B1

This study explored the molecular mechanisms underlying the time-dependent autophagy and apoptosis induced by nutrient depletion in human multiple myeloma cell line RPMI8226 cells. RT-PCR and qRT-PCR were used to evaluate the transcriptional levels of Deptor, JNK1, JNK2, JNK3, Raf-1, p53, p21 and NF kappa B1 at 0, 6, 12, 18, 24 and 48 h after nutrient depletion in RPMI8226 cells. We found that transcriptional levels of Deptor were increased time-dependently at 0, 6, 12 and 18 h, and then decreased. Its alternation was consistent with autophagy. Transcriptional levels of Raf-1, JNK1, JNK2, p53 and p21 were increased time-dependently at 0, 6, 12, 18,24 and 48 h accompanying with the increase of apoptosis. Transcriptional levels of NF kappa B1 at 6, 12, 18, 24 and 48 h were decreased as compared with 0 h. It was suggested that all the studied signaling molecules were involved in cellular response to nutrient depletion in RPMI8226 cells. Deptor contributed to autophagy in this process. Raf-1/JNK /p53/p21 pathway may be involved in apoptosis, and NF kappa B1 may play a possible role in inhibiting apoptosis. It remained to be studied whether Deptor was involved in both autophagy and apoptosis.

• 出版日期2012-2
• 单位华中科技大学