An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination

作者：Ruiz Hernandez Raul; Peroval Marylene; Boyd Amy; Balkissoon Devanand; Staines Karen; Smith Adrian; Butter Colin^*

来源：Journal of Immunological Methods, 2015, 416: 40-48.

DOI：10.1016/j.jim.2014.10.012

• Adaptive antiviral responses
• Antigen presentation
• Avian influenza
• IFN gamma ELISpot
• CD8 T cell

A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFN gamma ELISpot assay to enumerate ex vivo responses against influenza virus antigens. Splenocytes from birds challenged with influenza showed specific responses to the influenza virus, with responding cells being mainly CD8 positive. The utility of the assay was also demonstrated in the detection of an antigen specific enhancement of IFN gamma producing cells from birds vaccinated with recombinant Fowlpox vectored influenza nucleoprotein and matrix protein.

• 出版日期2015-1