DISTURBANCE OF CELL PROLIFERATION IN RESPONSE TO MOBILE PHONE FREQUENCY RADIATION

作者：Trosic Ivancica; Pavicic Ivan

来源：Arhiv za Higijenu Rada i Toksikologiju-Archives of Industrial Hygiene and Toxicology, 2009, 60(1): 109-115.

DOI：10.2478/10004-1254-60-2009-1891

• continuous cell culture
• microtbule structure
• mitotic index
• RF/MW
• 935 MHz

The aim of study was to determine the influence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79 cells after 1 h, 2 h, and 3 h of exposure. V79 cells were cultured in standard laboratory conditions and exposed to continuous-wave (CW) RF/MW radiation of 935 MHz, electric field strength of (8.2 /- 0.3) V m(-1), and specific absorption rate (SAR) of 0.12 W kg(-1). To identify proliferation kinetics, the cells were counted for each hour of exposure 24 h, 48 h, 72 h, and 96 h after respective exposures. Microtubule proteins were determined using specific immunocytochemical methods. Cell smears were analysed under a fluorescent microscope. The study included negative and positive controls. Mitotic index was determined by estimating the number of dividing cells 24 h after exposure and dividing it with the total number of cells. In comparison to the controls, cell proliferation declined in cells exposed for three hours 72 h after irradiation (p<0.05). Microtubule structure was clearly altered immediately after three hours of irradiation (p<0.05). The mitotic index in RF/MW-exposed cells did not differ from negative controls. However, even if exposure did not affect the number of dividing cells, it may have slowed down cell division kinetics as a consequence of microtubule impairment immediately after exposure.

• 出版日期2009-3
• 单位河北医科大学