Determination of 6 Selenium Species in Selenium-Enriched Food by Hyphenated Technique of High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry

作者：Wang Xin^*; Xing Yuan Na; Chen Ze Yong; Liang Bo

来源：Chinese Journal of Analytical Chemistry, 2013, 41(11): 1669-1674.

DOI：10.3724/SP.J.1096.2013.30491

• High performance liquid chromatography
• Inductively coupled plasma mass spectrometry
• Toys
• Selenium-enriched food
• Selenium species

A method 14 the determination of 6 selenium species in selenium-enriched food by hyphenated technique of High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP-MS) has been developed. The 6 selenium species were separated on a Hamilton PRP-X100 analysis column using 20 mmol/L citric acid as the mobile phase with pH 6.0 under gradient elution within 15 min. This method was easy to operate and with remarkably high sensitivity. The detection limits of SeO42-, SeO32-, selenomethionine (SeMet), selenocystine (SeCys(2)), selenourea (SeUr) and selenoethionine (SeEt) were found to be 0.7, 0.5, 4.1, 1.3, 13.6, 11.9 mu g/L for 100 mu/L injection volume in He collision cell mode of mass spectrometry. Selenium-enriched corn flour containing SeMet was used to investigate the results of different extraction methods. This method has been used to determine selenium species in some kinds of selenium-enriched food, including rice, yeast, tea and ganoderma lucidum spore powder. The results were satisfactory.

• 出版日期2013-11
• 单位深圳市计量质量检测研究院