Dependency of gamma-secretase complex activity on the structural integrity of the bilayer

作者：Zhou Hua^*; Zhou Shuxia; Walian Peter J; Jap Bing K

来源：Biochemical and Biophysical Research Communications, 2010, 402(2): 291-296.

DOI：10.1016/j.bbrc.2010.10.017

• Membrane protein
• Aspartyl protease
• Alzheimer's disease

gamma-secretase is a membrane protein complex associated with the production of All peptides that are pathogenic in Alzheimer's disease. We have characterized the activity of gamma-secretase complexes under a variety of detergent solubilization and reconstitution conditions, and the structural state of proteoliposomes by electron microscopy. We found that gamma-secretase activity is highly dependent on the physical state or integrity of the membrane bilayer - partial solubilization may increase activity while complete solubilization will abolish it. The activity of well-solubilized gamma-secretase can be restored to near native levels when properly reconstituted into a lipid bilayer environment.

• 出版日期2010-11-12