A highly selective "off-on" fluorescent sensor for subcellular visualization of labile iron(III) in living cells

作者：Ozdemir Mecit; Zhang Yi; Guo Maolin^*

来源：Inorganic Chemistry Communications, 2018, 90: 73-77.

DOI：10.1016/j.inoche.2018.02.015

• Fluorescence
• Sensor
• Ferric ion (Fe3+)
• Rhodamine
• Bioimaging
• Living cells

A highly selective fluorescent sensor "RhHPA" derived from a condensation reaction between rhodamine hydrizine and 6-hydroxymetyl-pyridine-2-aldehyde has been developed for selective Fe3+-imaging in living cells. The sensor exhibits marked selectivity and sensitivity towards Fe3+, with a chelation-induced "off-on" response. The sensor binds to Fe3+ at 1:1 stoichiometry with high affinity (apparent binding constant 1.27 x 10(7) M-1) and displays a distinct change in colour and fluorescence upon the alteration of free Fe3+ levels with a reversible response and little interference with other biological relevant metal ions. The sensor works at pH 5-9 that covers most of the physiological pH range. Cell imaging studies demonstrated that the sensor is capable of detecting basal level Fe3+ as well as the dynamic changes in Fe3+ levels in live cells at subcellular resolution, with two labile Fe3+ pools identified in mitochondria and endosomes/lysosomes in bovine aortic endothelial cells (BAEC) for the first time. Published by Elsevier B.V.

• 出版日期2018-4