The antiproliferative and apoptotic effects of apigenin on glioblastoma cells

作者：Stump Trevor A; Santee Brittany N; Williams Lauren P; Kunze Rachel A; Heinze Chelsae E; Huseman Eric D; Gryka Rebecca J; Simpson Denise S; Amos Samson

来源：Journal of Pharmacy and Pharmacology, 2017, 69(7): 907-916.

DOI：10.1111/jphp.12718

• apigenin
• apoptosis
• epidermal growth factor receptor
• glioblastoma
• proliferation

ObjectivesGlioblastoma (GBM) is highly proliferative, infiltrative, malignant and the most deadly form of brain tumour. The epidermal growth factor receptor (EGFR) is overexpressed, amplified and mutated in GBM and has been shown to play key and important roles in the proliferation, growth and survival of this tumour. The goal of our study was to investigate the antiproliferative, apoptotic and molecular effects of apigenin in GBM.
MethodsProliferation and viability tests were carried out using the trypan blue exclusion, MTT and lactate dehydrogenase (LDH) assays. Flow cytometry was used to examine the effects of apigenin on the cell cycle check-points. In addition, we determined the effects of apigenin on EGFR-mediated signalling pathways by Western blot analyses.
Key findingsOur results showed that apigenin reduced cell viability and proliferation in a dose- and time-dependent manner while increasing cytotoxicity in GBM cells. Treatment with apigenin-induced is poly ADP-ribose polymerase (PARP) cleavage and caused cell cycle arrest at the G2M checkpoint. Furthermore, our data revealed that apigenin inhibited EGFR-mediated phosphorylation of mitogen-activated protein kinase (MAPK), AKT and mammalian target of rapamycin (mTOR) signalling pathways and attenuated the expression of Bcl-xL.
ConclusionOur results demonstrated that apigenin has potent inhibitory effects on pathways involved in GBM proliferation and survival and could potentially be used as a therapeutic agent for GBM.

• 出版日期2017-7