Analysis of fullerene-based nanomaterial in serum matrix by CE

作者：Chan King C^*; Patri Anil K; Veenstra Timothy D; McNeil Scott E; Issaq Haleem J

来源：Electrophoresis, 2007, 28(10): 1518-1524.

DOI：10.1002/elps.200600724

• CE
• fullerenes
• nanotechnology

With the increasing interest in using nanoparticles as vehicles for drug delivery and image contrast agents, there is a need to develop assays for their detection and quantitation in complex matrices to facilitate monitoring their biodistribution. In this study, we developed a CE approach for the analysis of two nanoparticles: carboxyfullerene (C3) and dendrofullerene (DF1) in both standard solutions and a serum matrix. These highly soluble, charged C-60 derivatives were characterized by CZE using either a bare or dynamically coated fused-silica capillaries. The resolution of both nanoparticles was slightly lower with the coated capillary; however, their migration times were faster. While separation of the DF1 nanoparticles using MEKC resulted in a greater number of observable peaks, the peak profile of C3 was basically unchanged regardless of whether SDS micelles were added to the running buffers or not. The MEKC and/or CZE assays were then used to quantitate the C3 and DF1 nanoparticles in spiked human serum samples. The quantitation of the nanoparticles was linear from 0-500 mu g/mL with detection limits ranging from 0.5 to 6 mu g/mL.

• 出版日期2007-5