Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS I. Sample preparation

作者：Bischoff Rainer^*; Bronsema Kees J; van de Merbel Nico C

来源：TrAC-Trends in Analytical Chemistry, 2013, 48: 41-51.

DOI：10.1016/j.trac.2012.11.015

• Analytical procedure
• Automation
• Biological matrix
• Biopharmaceuticals
• Blood-derived body fluid
• Clinical study
• Liquid chromatography with tandem mass spectrometry (LC-MS/MS)
• Pharmacokinetic study
• Protein
• Sample preparation

Part I of this review discusses sample-preparation aspects of quantifying biopharmaceutical proteins in complex biological matrices by LC-MS/MS with a focus on blood-derived body fluids. We conclude Part I with a short overview over options for automating the entire analytical procedure, which is indispensable for routine applications in pharmacokinetic and clinical studies.

• 出版日期2013-8