Antibacterial effect and hydrophobicity of yak kappa-casein hydrolysate and its fractions

作者：Cheng X; Tang X; Wang Q; Mao X Y^*

来源：International Dairy Journal, 2013, 31(2): 111-116.

DOI：10.1016/j.idairyj.2012.12.004

• Antibacterial effects
• Casein hydrolysate
• Gel filtration
• Monocyte-macrophages
• Potential inhibitors
• Raw 264.7 cells
• Sephadex
• Surface hydrophobicity

The antibacterial effects of yak kappa-casein hydrolysate and its fractions on Escherichia coli were determined. Their surface hydrophobicity and amino acid profiles were also evaluated. Compared with intact yak kappa-casein, yak kappa-casein hydrolysate obtained with trypsin (YCHT) over 4 h of hydrolysis, permeate of YCHT obtained with 10 kDa cut-off membrane and permeate-F2 obtained from Sephadex G-25 gel filtration of permeate, exhibited a significantly higher antibacterial effect. Compared with YCHT and its permeate, permeate-F2 showed higher surface hydrophobicity when using PRODAN as a probe. Compared with native kappa-casein, YCHT and permeate-F2 have a higher content and ratio of Lys, Pro, Phe and Leu residues. In contrast to its antibacterial effect, YCHT showed no cytotoxicity to mouse leukaemic monocyte macrophage RAW 264.7 cells at the concentrations used from 0 mg mL(-1) to 4 mg mL(-1). Therefore, yak kappa-casein hydrolysate and its fractions may be used as potential inhibitors of E. coli.

• 出版日期2013-8
• 单位中国农业大学