A novel label-free colorimetric detection of L-histidine using Cu2+-modulated G-quadruplex-based DNAzymes

作者：Gu, Pan; Zhang, Gehou; Deng, Zhiyi; Tang, Zhenwei; Zhang, Huifang; Khu**u, Farjana Yeasmin; Wu, Kefeng; Chen, Mingjian; Ma, Changbei^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 203: 195-200.

DOI：10.1016/j.saa.2018.05.084

• Histidine
• Colorimetric
• DNAzyme
• G-quadruplex

We proposed a colorimetric method for L-histidine detection based on Cu2+-mediated DNAzyme and G-quadruplex-hemin complex catalyzed oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). In this system, after the addition of L-histidine, the formation of G-quadruplex-hemin complex will be disturbed, thus the colorimetric signal intensity conversely corresponds to the concentration of histidine. In this assay, a lower detection limit of L-histidine (50 nM) is addressed comparing to previously reported colorimetric methods. The cost is extremely low as the proposed design is both label-free and enzyme free. All the more vitally, the colorimetric detection procedure is substantially straightforward with no further modification processes. By and large, the sensor can provide a promising plan for the detection of L-histidine.

• 出版日期2018-10-5
• 单位中南大学