microRNA signatures in peripheral blood fail to detect acute cellular rejection after liver transplantation

作者：Raschzok Nathanael; Reutzel Selke Anja; Schmuck Rosa B; Tannus Laura M; Morgul Mehmet H; Dietel Corinna; Leder Annekatrin; Struecker Benjamin; Lippert Steffen; Sallmon Hannes; Schmelzle Moritz; Bartels Michael; Jonas Sven; Pratschke Johann; Sauer Igor M^*

来源：Biomarkers, 2016, 21(8): 699-707.

DOI：10.3109/1354750X.2016.1172113

• Allograft
• biomarker
• Paxgene

Objective: We investigated whether microRNA signatures in whole blood samples are associated with acute cellular rejection (ACR) after liver transplantation.
Materials and methods: Blood samples were collected using Paxgene technology and analyzed by microarrays and quantitative real-time polymerase chain reaction (qRT-PCR).
Results: microRNA signatures failed to distinguish between 19 patients with ACR and 16 controls. Let-7b-5p and let-7c were upregulated in a subgroup of patients with ACR during the 6th and 7th postoperative days but failed in an independent validation of 20 patients.
Conclusion: microRNA signatures in whole blood processed by Paxgene technology are not suited for the detection of ACR after liver transplantation.

• 出版日期2016