Application of sugaring-out extraction for the determination of sulfonamides in honey by high-performance liquid chromatography with fluorescence detection

作者：Tsai Wen Hsien; Chuang Hung Yi; Chen Ho Hsien; Wu Yuh Wern; Cheng Shou Hsun; Huang Tzou Chi^*

来源：Journal of Chromatography A, 2010, 1217(49): 7812-7815.

DOI：10.1016/j.chroma.2010.10.008

• Sample preparation
• Sugaring out assisted liquid-liquid extraction
• Honey
• Sulfonamides
• Fluorescamine derivatization

A simple sugaring-out assisted liquid-liquid extraction method combined with high-performance liquid-chromatography with fluorescence detection (HPLC-FL) was developed for the extraction and determination of sulfonamides in honey Sample preparation consisted of acid hydrolysis to release sugar-bound sulfonamides After derivatization with fluorescamine the derivatives were partitioned into the organic layer under the honey (sugar)/water/acetonitrile system The clear organic extract obtained by centrifugation could be injected Into the HPLC system either directly or after dilution Linearity was obtained with the coefficient of determination (R(2)) higher than 0 998 from 2 to 200 ng/mL Under the optimal conditions recoveries were determined for honey fortified at three levels (5 20 an

• 出版日期2010-12-3